SIRT1 antibody (AA 1-350)

Catalog No. ABIN7119109

This Mouse Monoclonal antibody specifically detects SIRT1 in WB, IHC, ELISA and IP. It exhibits reactivity toward Human.

Quick Overview for SIRT1 antibody (AA 1-350) (ABIN7119109)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Clone: 6A1

Product Details anti-SIRT1 Antibody

Binding Specificity: AA 1-350

Purpose: SIRT1 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 1-350aa

Immunogen: sirtuin(silent mating type information regulation 2 homolog) 1

Isotype: IgG1

Application Details

Application Notes: WB: 1:2000-1:5000, IP: 1:500-1:2000 for WB, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1

Background:

Synonyms: NAD-dependent protein deacetylase sirtuin-1 (hSIRT1)|NAD-dependent protein deacylase sirtuin-1|Regulatory protein SIR2 homolog 1|SIR2-like protein 1 (hSIR2)|SirtT1 75 kDa fragment (75SirT1)|SIRT1|SIR2L1

Background: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.

Molecular Weight: 130 kDa

Gene ID: 23411

UniProt: Q96EB6

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling